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First, to a 1.5-mL Eppendorf tube were added 25 L of GSH (12 mM), 45 L of CDNB (3.0 mM), 50 L of GST (appropriate concentration), and 200 L of triethanolamineHCl buffer (20 mM, pH 6.5), and then, the mixed solution was incubated for 60 min at 25 C

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