Immunofluorescence and immunohistochemical assay After dewaxing and hydration, the tissue sections were immersed in PBS for 5 min, and then the sample areas were circled using a histochemical pen

Dietary influences are the most common cause of stool colour variation: Green stools : leafy vegetables (spinach, kale), green food colouring Red or reddish stools : beetroot, tomatoes, red food dyes, cranberries Orange stools : foods high in beta-carotene (carrots, sweet potatoes), rifampicin (an antibiotic) Dark brown to black : iron supplements, bismuth subsalicylate, liquorice, blueberries Pale or light-coloured : primarily caused by reduced bile flow into the intestine Medications and supplements frequently alter stool appearance: Iron supplements commonly cause dark green or black stools Rifampicin and rifabutin can produce orange-red discolouration Bismuth subsalicylate causes black stools Some vitamins and herbal supplements contain pigments that pass through the digestive system Medical conditions that may affect stool colour include: Pale/clay-coloured : biliary obstruction, hepatitis, cirrhosis, pancreatic disorders Black/tarry : upper gastrointestinal bleeding (peptic ulcers, gastritis) Bright red : lower gastrointestinal bleeding (haemorrhoids, diverticular disease, colorectal pathology) Yellow/greasy : malabsorption syndromes, coeliac disease, chronic pancreatitis Green : rapid intestinal transit, certain infections Patients commencing GLP-1 therapy often simultaneously modify their diet significantly, increasing vegetable intake and reducing processed foods as part of weight management efforts

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Lifestyle interventions, including exercise and dietary modification, remain the cornerstone of obesity and diabetes management, with proven benefits for weight loss, glycemic control, cardiovascular health, and quality of life (11, 16, 18, 30)
Endorsed by #ATTD and several leading diabetes organizations, the consensus provides practical recommendations to support clinicians in the safe and effective use of these therapies, including guidance on insulin adjustments, risk mitigation, and clinical decision-making
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