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The overnight culture (2030 ml) was added to 1,000 ml of 2YT medium (containing 100 g/ml carbenicillin and 50 g/ml kanamycin) up to an optical density at 600 nm (OD 600 ) = 0.1 and grown at 37C until OD 600 = 0.6 before induction with 1 mM isopropyl--D-1- thiogalactopyranoside

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Gacesa R, Kurilshikov A, Vich Vila A, Sinha T, Klaassen MAY, Bolte LA

Figure 2 Oligomeric A 40 Depletes Endogenous GSH Levels Which Can be Attenuated Using the GGC Oligomeric A 40 decreased the activity of the antioxidant enzymes SOD (Figure 3A) and GPx (Figure 3B) compared to non-treated cells

Persulfide signaling in stress-initiated calmodulin kinase response