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Regulation of epithelial proliferation by tight junction proteins

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Phan TT, Abozguia K, Shivu GN, Mahadevan G, Ahmed I, Williams L, et al

Cells were directly lysed in Laemmli buffer (60 mM Tris-HCl (pH 6.8), 2% (w/v) sodium dodecyl sulfate (SDS), 10% (v/v) glycerol, and 0.02% (w/v) bromophenol blue), then sonicated and denatured by boiling at 95 for 5 minutes

Elevating the head of the bed 6 to 8 inches provides overnight gravitational protection

Hence, the radiotherapy was suspended