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For protein purification, the cell pellet was resuspended in 120 mL of the lysis buffer (50 mM Na 2 HPO 4 , pH 8.0, 500 mM NaCl and 30 mM imidazole), and affinity chromatography was performed using a 35 mL Omnifit glass column packed with Ni Sepharose 6 Fast Flow (Cytiva)