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Immunofluorescence (a) and quantification (b,c) of cFos positive neurons in the ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH) (n = 4 male 47-wk old C57BL/6 J mice each group, scale bar: 100 m)

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These results prompted the hypothesis on a direct role of GLP-1 in controlling pubertal development through the hypothalamic GnRH secretion, although this mechanism still remains to be unraveled