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It was further shown that NPS applied intracerebroventricularly was able to reduce airway responsiveness to methacholine in experimental animals, most likely through a CNS-mediated pathway connecting respiratory and stress responses [35]

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Briefly, cells were lysed using the RIP lysis buffer provided in the kit, and cell lysates were incubated with magnetic beads pre-coated with either anti-SYNCRIP antibody (R5653, Sigma-Aldrich) or purified mouse IgG (5415, Cell Signaling Technology) as a negative control

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