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On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)

doi:10.1016/j.burns.2014.06.001

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doi:10.1001/jama.2019.18714

CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat

Use standard PPE and dispose of materials in accordance with institutional waste procedures