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Oil Red O staining On day 6 of differentiation, intramuscular adipocytes were washed three times with PBS and fixed in 4% paraformaldehyde for 30 min at room temperature

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doi: 10.3390/nu12061567 23 YangMShenXLuDPengJZhouSXuLet al

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Progesterone's metabolite allopregnanolone acts on the same GABA-A receptors targeted by benzodiazepines when progesterone falls in perimenopause, this endogenous calming signal weakens, leaving the nervous system structurally less buffered against stress

Genghof 1970