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The animals were placed in the stereotaxic device (David Kopf Instruments, Tujunga, CA, USA) and holes were drilled in the frontal, parietal, and occipital regions of the skull at the following locations: 2 mm from Bregma, 2 mm lateral to the midline (ipsilateral), and 2 mm posterior to Lambda (reference electrode)
Consistent with human observations, H2O2 also promoted FOXO4 nuclear translocation in TM3 cells, along with an increase in senescence markers p53, phospho-p53, and p21, while reducing testosterone synthesis-related enzymes like CYP11A1 and CYP17A1
2015 Jul 22;6:370
D., Hol, E
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