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Lets say that your target protein is smaller than GST, such that most of the size of the GST-fusion protein will be contributed from GST and not your target protein

Thyroid 28(4):445453 De Leo S, Trevisan M, Moneta C et al (2023) Endocrine-related adverse conditions induced by tyrosine kinase inhibitors

[DOI] [PMC free article] [PubMed] [Google Scholar] Cave M.C., Hurt R.T., Frazier T.H., Matheson P.J., Garrison R.N., McClain C.J., McClave S.A

For venetoclax alone, a median of 82.2% of cells died, and the percentage was similar (85.9%) after the addition of CB-839 (P = 0.28)

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