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Identification of an important cysteine residue in human glutamate-cysteine ligase catalytic subunit by site-directed mutagenesis

View Source ORTHOP J SPORTS MED BPC-157 in Orthopaedic Sports Medicine Systematic Review 2025 Vasireddi N, Hahamyan H, Salata MJ, et al

Comprehensive toxicology studies found no lethal dose even at extremely high levels (20 mg/kg intramuscularly in rats, equivalent to approximately 1,400 mg for a 155 pound human)

The copper-peptide bond that makes GHK-Cu effective for tissue repair and collagen synthesis is also what makes it temperature-sensitive after mixing