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After 1 mM CaCl 2 was added for Tse3-Tsi3 protein interaction, the supernatant was then loaded to the CNBr-activated Sepharose resin (GE Healthcare) conjugated with Tse3 protein by gravity flow

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In men, it often leads to a receding hairline and baldness at the crown, while in women, it results in thinning of the hair on the crown and widening of the part line