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GPR146-deficient mice exhibit elevated energy expenditure To investigate whether altered energy utilization contributes to the lean phenotype of Gpr146 / mice, and to distinguish potential causal mechanisms from secondary adaptations, we performed indirect calorimetry 27,28 after 2 weeks of HFD feeding, a time point when fat mass differences between Gpr146 / mice and their wild-type littermates begin to emerge (Fig

C4OH, C14 and C16:1 were significantly elevated as compared with the upper limit of normal ( z =2.18, P =0.01

These considerations argue for a more nuanced evaluation of centrally acting appetite peptides rather than categorical exclusion

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