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Peptides were then separated across a 5 101 mm Betasil C8 column (Thermo Fisher Scientific) with a linear gradient of 1240% acetonitrile in 0.3% formic acid over a short five minute gradient to limit back exchange with the solvent

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The ever-expanding role of degradation in the regulation of apolipoprotein B metabolism J Lipid Res

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This suggests that not all individuals with ASD may have abnormal digestion of gluten and casein leading to opioid peptides