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Images were captured through 63 and 100 oil-immersion objectives for the following fluorophores: DAPI (405-nm laser excitation, 445/40-nm emission), AlexaFluor 488 (491-nm laser excitation, 528/38-nm emission), PE and AlexaFluor 568 (561-nm laser excitation, 617/73-nm emission) and AlexaFluor 647 (647-nm laser excitation, 700/75-nm emission)

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Knowing when and how to use it especially around the weekly rhythm of injections is where most articles fail you entirely

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