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Li Manni G, Alavi A (2018) Understanding the mechanism stabilizing intermediate spin states in Fe(II)-porphyrin

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For fixed imaging, worms were first washed three times with 1 mL of 0.1% Tween in 1 PBS, resuspended in 1 mL of 100% methanol, and incubated at 30 C for 5 min

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After that, the beads in the capsule dissolve over a period of 8 hours to slowly release all the nutrients